Inhibition of hepatitis B virus surface gene expression by antisense oligodeoxynucleotides in a human hepatoma cell line.
Identifieur interne : 004520 ( Main/Exploration ); précédent : 004519; suivant : 004521Inhibition of hepatitis B virus surface gene expression by antisense oligodeoxynucleotides in a human hepatoma cell line.
Auteurs : M. Reinis [République tchèque] ; M. Reinisová ; E. Korec ; I. HlozánekSource :
- Folia biologica [ 0015-5500 ] ; 1993.
Descripteurs français
- KwdFr :
- ARN messager (métabolisme), Antigènes de surface du virus de l'hépatite B (génétique), Antigènes de surface du virus de l'hépatite B (métabolisme), Carcinome hépatocellulaire (microbiologie), Données de séquences moléculaires, Gènes viraux (), Humains, Lignée cellulaire, Oligonucléotides antisens (pharmacologie), Protéines virales structurales (génétique), Régulation de l'expression des gènes viraux (), Séquence nucléotidique, Test ELISA, Virus de l'hépatite B (génétique).
- MESH :
- génétique : Antigènes de surface du virus de l'hépatite B, Protéines virales structurales, Virus de l'hépatite B.
- microbiologie : Carcinome hépatocellulaire.
- métabolisme : ARN messager, Antigènes de surface du virus de l'hépatite B.
- pharmacologie : Oligonucléotides antisens.
- Données de séquences moléculaires, Gènes viraux, Humains, Lignée cellulaire, Régulation de l'expression des gènes viraux, Séquence nucléotidique, Test ELISA.
English descriptors
- KwdEn :
- Base Sequence, Carcinoma, Hepatocellular (microbiology), Cell Line, Enzyme-Linked Immunosorbent Assay, Gene Expression Regulation, Viral (drug effects), Genes, Viral (drug effects), Hepatitis B Surface Antigens (genetics), Hepatitis B Surface Antigens (metabolism), Hepatitis B virus (genetics), Humans, Molecular Sequence Data, Oligonucleotides, Antisense (pharmacology), RNA, Messenger (metabolism), Viral Structural Proteins (genetics).
- MESH :
- chemical , genetics : Hepatitis B Surface Antigens, Viral Structural Proteins.
- drug effects : Gene Expression Regulation, Viral, Genes, Viral.
- genetics : Hepatitis B virus.
- chemical , metabolism : Hepatitis B Surface Antigens, RNA, Messenger.
- microbiology : Carcinoma, Hepatocellular.
- chemical , pharmacology : Oligonucleotides, Antisense.
- Base Sequence, Cell Line, Enzyme-Linked Immunosorbent Assay, Humans, Molecular Sequence Data.
Abstract
We have studied the inhibitory effect of antisense oligodeoxynucleotides on the expression of hepatitis B virus surface antigens. Human hepatoma cell line PLC/PRF/5 harbors several integrated copies of the HBV genome and produces and secretes hepatitis B virus surface antigen (HBsAg) to the medium. Synthetic antisense oligodeoxynucleotides complementary to various regions of the surface antigen gene were synthesized and their ability to block its expression was tested. Oligodeoxynucleotides (17- and 21-mers) complementary to regions covering ATG codons of both preS2 and S genes significantly inhibited preS2 and S protein production. Less efficient inhibition was achieved when the oligonucleotide complementary to the inside S gene region was assayed.
PubMed: 8206172
Affiliations:
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Le document en format XML
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Korec</name></author><author><name sortKey="Hlozanek, I" sort="Hlozanek, I" uniqKey="Hlozanek I" first="I" last="Hlozánek">I. 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Reinis</name><affiliation wicri:level="3"><nlm:affiliation>Institute of Molecular Genetics, Academy of Sciences of Czech Republic, Praha.</nlm:affiliation><country>République tchèque</country><placeName><settlement type="city">Prague</settlement><region type="région" nuts="2">Bohême centrale</region></placeName><wicri:orgArea>Institute of Molecular Genetics, Academy of Sciences of Czech Republic</wicri:orgArea></affiliation></author><author><name sortKey="Reinisova, M" sort="Reinisova, M" uniqKey="Reinisova M" first="M" last="Reinisová">M. Reinisová</name></author><author><name sortKey="Korec, E" sort="Korec, E" uniqKey="Korec E" first="E" last="Korec">E. Korec</name></author><author><name sortKey="Hlozanek, I" sort="Hlozanek, I" uniqKey="Hlozanek I" first="I" last="Hlozánek">I. Hlozánek</name></author></analytic><series><title level="j">Folia biologica</title><idno type="ISSN">0015-5500</idno><imprint><date when="1993" type="published">1993</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Carcinoma, Hepatocellular (microbiology)</term><term>Cell Line</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Gene Expression Regulation, Viral (drug effects)</term><term>Genes, Viral (drug effects)</term><term>Hepatitis B Surface Antigens (genetics)</term><term>Hepatitis B Surface Antigens (metabolism)</term><term>Hepatitis B virus (genetics)</term><term>Humans</term><term>Molecular Sequence Data</term><term>Oligonucleotides, Antisense (pharmacology)</term><term>RNA, Messenger (metabolism)</term><term>Viral Structural Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (métabolisme)</term><term>Antigènes de surface du virus de l'hépatite B (génétique)</term><term>Antigènes de surface du virus de l'hépatite B (métabolisme)</term><term>Carcinome hépatocellulaire (microbiologie)</term><term>Données de séquences moléculaires</term><term>Gènes viraux ()</term><term>Humains</term><term>Lignée cellulaire</term><term>Oligonucléotides antisens (pharmacologie)</term><term>Protéines virales structurales (génétique)</term><term>Régulation de l'expression des gènes viraux ()</term><term>Séquence nucléotidique</term><term>Test ELISA</term><term>Virus de l'hépatite B (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Hepatitis B Surface Antigens</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression Regulation, Viral</term><term>Genes, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Hepatitis B virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes de surface du virus de l'hépatite B</term><term>Protéines virales structurales</term><term>Virus de l'hépatite B</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Hepatitis B Surface Antigens</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Carcinome hépatocellulaire</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Carcinoma, Hepatocellular</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term><term>Antigènes de surface du virus de l'hépatite B</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Oligonucléotides antisens</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Oligonucleotides, Antisense</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Cell Line</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Gènes viraux</term><term>Humains</term><term>Lignée cellulaire</term><term>Régulation de l'expression des gènes viraux</term><term>Séquence nucléotidique</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have studied the inhibitory effect of antisense oligodeoxynucleotides on the expression of hepatitis B virus surface antigens. Human hepatoma cell line PLC/PRF/5 harbors several integrated copies of the HBV genome and produces and secretes hepatitis B virus surface antigen (HBsAg) to the medium. Synthetic antisense oligodeoxynucleotides complementary to various regions of the surface antigen gene were synthesized and their ability to block its expression was tested. Oligodeoxynucleotides (17- and 21-mers) complementary to regions covering ATG codons of both preS2 and S genes significantly inhibited preS2 and S protein production. Less efficient inhibition was achieved when the oligonucleotide complementary to the inside S gene region was assayed.</div></front></TEI><affiliations><list><country><li>République tchèque</li></country><region><li>Bohême centrale</li></region><settlement><li>Prague</li></settlement></list><tree><noCountry><name sortKey="Hlozanek, I" sort="Hlozanek, I" uniqKey="Hlozanek I" first="I" last="Hlozánek">I. Hlozánek</name><name sortKey="Korec, E" sort="Korec, E" uniqKey="Korec E" first="E" last="Korec">E. Korec</name><name sortKey="Reinisova, M" sort="Reinisova, M" uniqKey="Reinisova M" first="M" last="Reinisová">M. Reinisová</name></noCountry><country name="République tchèque"><region name="Bohême centrale"><name sortKey="Reinis, M" sort="Reinis, M" uniqKey="Reinis M" first="M" last="Reinis">M. Reinis</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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